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Recombinant Human Fc gamma RIIB/FCGR2B/CD32b Protein (RP00075)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human Fc gamma RIIB/FCGR2B/CD32b Protein
Catalog No.RP00075
DescriptionRecombinant Human Fc gamma RIIB/FCGR2B/CD32b Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ala46-Pro217) of human Fc gamma RIIB/C (Accession #NP_003992.3) fused with a 6×His tag at the C-terminus.
Bio-Activity1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human FCGR2B at 2 μg/mL (100 μL/well) can bind biotinylated Human IgG1 with a linear range of 0.0048-2.81 μg/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human FCGR2B (Catalog:RP00075) at 2μg/mL (100 μL/well) can bind FCGR2B Rabbit pAb (Catalog: A12553) with a linear range of 0.3-77.48ng/mL.
Purity> 97% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW20.17 kDa
The protein is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells.
SynonymFCGR2B; CD32; CD32B; FCG2; FCGR2; IGFR2
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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