Overview
| Product name | Recombinant Human IL-12B/IL-12 p40 Protein |
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| Catalog No. | RP01288 |
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| Description | Recombinant Human IL-12B/IL-12 p40 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ile23-Ser328) of human IL-12B/P40 (Accession #NP_002178.2) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by its binding ability in a functional ELISA.Immobilized Human IL12B (Catalog: RP01288) at 5 μg/mL (100 μL/well) can bind Human IL12RB1 with a linear range of 0.02-2.7 ng/mL.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 35.54 kDa |
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Product information
| Synonym | IL12B; CLMF; CLMF2; IL-12B; IMD28; IMD29; NKSF; NKSF2 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
