제품 > 항체 > 단일클론항체(mAb)

IL12B Rabbit mAb (A23577)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IL12B Rabbit mAb (A23577)

Western blot analysis of various lysates, using IL12B Rabbit mAb (A23577) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - IL12B Rabbit mAb (A23577)

Immunofluorescence analysis of paraffin-embedded Rat thymus tissue using IL12B Rabbit mAb(A23577) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - IL12B Rabbit mAb (A23577)

Immunofluorescence analysis of paraffin-embedded Mouse thymus tissue using IL12B Rabbit mAb(A23577) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - IL12B Rabbit mAb (A23577)

Immunofluorescence analysis of paraffin-embedded Human tonsil tissue using IL12B Rabbit mAb(A23577) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameIL12B Rabbit mAb
Catalog No.A23577
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC3090
This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 228-328 of human IL12B (NP_002178.2).
SequenceFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS
Gene ID
Swiss Prot
SynonymsCLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B; IL12B
Calculated MW37kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, U-251MG, Mouse thymus, Mouse spleen, Rat thymus, Rat spleen
Cellular locationSecreted.

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Certificate of Compliance

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ABclonal:Western blot - IL12B Rabbit mAb (A23577)}

Western blot - IL12B Rabbit mAb (A23577)

Western blot analysis of various lysates, using IL12B Rabbit mAb (A23577) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - IL12B Rabbit mAb (A23577)}

Immunofluorescence - IL12B Rabbit mAb (A23577)

Immunofluorescence analysis of paraffin-embedded Rat thymus tissue using IL12B Rabbit mAb(A23577) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - IL12B Rabbit mAb (A23577)}

Immunofluorescence - IL12B Rabbit mAb (A23577)

Immunofluorescence analysis of paraffin-embedded Mouse thymus tissue using IL12B Rabbit mAb(A23577) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - IL12B Rabbit mAb (A23577)}

Immunofluorescence - IL12B Rabbit mAb (A23577)

Immunofluorescence analysis of paraffin-embedded Human tonsil tissue using IL12B Rabbit mAb(A23577) at a dilution of 1:25 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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