Overview
| Product name | Recombinant Human NCAM-1/CD56 Protein |
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| Catalog No. | RP03107 |
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| Description | Recombinant Human NCAM-1/CD56 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu20-Gly718) of Human NCAM-1/CD56 (Accession #NP_851996.2) fused with a 6×His tag at the C-terminus. |
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| Bio-Activity | Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A Human neuroblastoma cells. The ED50 for this effect is 1.8-7.2 μg/mL.
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| Purity | > 95% as determined by HPLC |
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| Endotoxin | < 0.01 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Human |
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| Calculated MW | 78.23 kDa |
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Background
Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.
Product information
| Synonym | N-CAM-1; NCAM-1; NCAM1; CD56; NCAM; MSK39 |
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| Expression Host | HEK293 cells |
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| Tag | C-His |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
