Overview
| Product name | Recombinant Human PBEF/Visfatin/NAMPT Protein |
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| Catalog No. | RP00724 |
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| Description | Recombinant Human PBEF/Visfatin/NAMPT Protein is produced by E. coli expression system. The target protein is expressed with sequence (Asn2-His491) of human Visfatin/Nampt (Accession #P43490) fused with no additional amino acid. |
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| Bio-Activity | Measured in a cell proliferation assay using RPMI 8226 cells. The ED50 for this effect is 1.72‑6.89 ng/mL, corresponding to a specific activity of 1.45×105~5.81×105units/mg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, pH8.0.Contact us for customized product form or formulation. |
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| Species | Human |
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| Calculated MW | 55.39 kDa |
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Background
Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonalexpansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellularenzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “ visfatin ” . PBEF is constitutivelyexpressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular andextracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induceangiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has beenshown to increase inflammatory cytokines, such as TNF- α , IL-1 β , IL-16, and TGF- β 1. PBEF also increases theproduction of IL-6, TNF- α , and IL-1 β in CD14+ monocyctes, macrophages, and dendritic cells, enhances theeffectiveness of T cells.
Product information
| Synonym | NAMPT; 1110035O14Rik; PBEF; PBEF1; VF; VISFATIN |
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| Expression Host | E. coli |
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| Tag | NO-tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
