| Product name | Recombinant Human TNFSF9/4-1BB Ligand Protein |
|---|---|
| Catalog No. | RP00060 |
| Description | Recombinant Human TNFSF9/4-1BB Ligand Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg71-Glu254) of human 4-1BB Ligand/TNFSF9 (Accession #NP_003802.1) fused with a 6×His tag at the C-terminus. |
| Bio-Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized recombinant human TNFSF9 at 2 μg/mL (100 μL/well) can bind recombinant human TNFRSF9 with a linear range of 40-100 ng/mL. 2.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF9/4-1BB at 2 μg/mL (100 μL/well) can bind Human TNFRSF9/4-1BB/CD137 with a linear range of 0.1-5.8 ng/mL. |
| Purity | > 95% by SDS-PAGE. |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation | Lyophilized from a 0.22 μm filtered solution of 50mM Tris, 150mM NaCl, pH 8.0.Contact us for customized product form or formulation. |
| Species | Human |
| Calculated MW | 20.28 kDa |
| Synonym | 4-1BB-L; CD137L; TNLG5A; TNFSF9; CD137L; TNLG5A |
|---|---|
| Expression Host | E. coli |
| Tag | C-His |
| Swiss-Prot | |
| Gene ID |
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
|---|---|
| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
* For research use only. Not for therapeutic or diagnostic purposes.
