Overview
| Product name | Recombinant Human TNFSF9/4-1BB Ligand Trimer Protein |
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| Catalog No. | RP00360 |
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| Description | Recombinant Human TNFSF9/4-1BB Ligand Trimer Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg71-Glu254) of Human TNFSF9/4-1BB Ligand Trimer (Accession #P41273) fused with a N-monomeric hFc tag at the N-terminus. |
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| Purity | > 95% by SDS-PAGE |
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| Endotoxin | < 1 EU/μg of the protein by LAL method |
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| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
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| Species | Human |
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| Calculated MW | 84.3 kDa |
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Background
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Product information
| Synonym | 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9 |
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| Expression Host | HEK293 cells |
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| Tag | N-monomeric hFc |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
