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Recombinant Mouse IL-12B/IL-12 p40 Protein (RP01661)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Mouse IL-12B/IL-12 p40 Protein
Catalog No.RP01661
DescriptionRecombinant Mouse IL-12B/IL-12 p40 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met23-Ser335) of mouse IL-12B (Accession #NP_001152896.1) fused with and a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA.Immobilized Mouse IL12B (Catalog: RP01661) at 5 μg/mL (100 μL/well) can bind Human IL12RB1 with a linear range of 0.001-1.3 ng/mL.
Purity> 97% by SDS-PAGE.
Endotoxin<0.1EU/μg
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
SpeciesMouse
Calculated MW36.63 kDa
Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells and has a broad array of biological activities.
Synonymp40; Il-12b; Il12p40; Il-12p40; IL12B
Expression HostHEK293 cells
TagC-6his
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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