Recombinant Mouse IL-12B/IL-12 p40 Protein (RP01696)

Overview

Product name	Recombinant Mouse IL-12B/IL-12 p40 Protein
Catalog No.	RP01696
Description	Recombinant Mouse IL-12B/IL-12 p40 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met23-Ser335) of mouse IL-12B (Accession #NP_001152896.1) fused with no additional amino acid.
Bio-Activity	Measured by its binding ability in a functional ELISA.Immobilized Mouse IL12B (Catalog: RP01696) at 10 μg/mL (100 μL/well) can bind Human IL12RB1 with a linear range of 0.02-51 ng/mL.
Purity	> 92% by SDS-PAGE.
Endotoxin	<0.1EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	Mouse
Calculated MW	35.79 kDa

Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells and has a broad array of biological activities.IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine is important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen.

Product information

Synonym	p40; Il-12b; Il12p40; Il-12p40; IL12B
Expression Host	HEK293 cells
Tag	NO-tag
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.