Overview
| Product name | Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein |
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| Catalog No. | RP00666 |
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| Description | Recombinant Mouse IL-1Ra/IL-1F3/IL-1RN Protein is produced by E. coli expression system. The target protein is expressed with sequence (Arg27-Gln178) of mouse IL-1Ra/IL-1F3/IL-1RN (Accession #P25085) fused with an initial Met at the N-terminus. |
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| Bio-Activity | Measured by its ability to inhibit IL-1 alpha-dependent proliferation in D10.G4.1 mouse helper T cells. The ED50 for this effect is 2.47-9.86 ng/mL in the presence of 3ng/mL of recombinant human IL-1 alpha(Catalog: RP00098), corresponding to a specific activity of 1.01×105~4.05×105 units/mg.
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| Purity | > 95% by SDS-PAGE. |
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| Endotoxin | < 1 EU/μg of the protein by LAL method. |
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| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
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| Species | Mouse |
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| Calculated MW | 17.33 kDa |
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Background
Interleukin-1 receptor antagonist protein (Il1rn), also known as IL-1ra, IRAP or IL1 inhibitor, is a member of theinterleukin 1 cytokine family. This protein inhibits the activities of interleukin 1 alpha (IL1A) and interleukin 1beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. The mouseIl1rn gene encodes a 178 amino acids (aa) protein with a 26 aa signal peptide. Mouse Il1rn protein shares 26%and 19% identity with its homologues IL-1 beta and IL-1 alpha, respectively. Il1rn can Inhibits the activity ofinterleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP forsignaling, but has no interleukin-1 like activity. Recently, an recombinant human Il1rn protein is used in thetreatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.
Product information
| Synonym | IL-1ra; F630041P17Rik; IL1RN |
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| Expression Host | E. coli |
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| Tag | No tag |
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| Swiss-Prot | |
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| Gene ID | |
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Usage guide
| Storage | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
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| Reconstitution | Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
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* For research use only. Not for therapeutic or diagnostic purposes.
