Recombinant human Trk-A/NTRK1 Protein (RP01726)

Overview

Product name	Recombinant human Trk-A/NTRK1 Protein
Catalog No.	RP01726
Description	Recombinant human Trk-A/NTRK1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala33-Glu413) of human Trk-A/NTRK1 (Accession #NP_001012331.1) fused with and a 6×His tag at the C-terminus.
Bio-Activity	Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.086-0.34 µg/mL in the presence of 10 ng/mL of Recombinant Human beta-NGF.
Endotoxin	< 0.01EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Species	human
Calculated MW	42.35 kDa

This protein is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

Product information

Synonym	MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; NTRK1
Expression Host	HEK293 cells
Tag	C-6His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.