제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human TrkA mAb (A23974)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry:1X10^6 MOLT-4 cells (negative control, left) and SH-SY5Y cells (right) were surface-stained with TrkA Rabbit mAb(A23974, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb (1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human TrkA mAb(A23974, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human TrkA mAb
Catalog No.	A23974
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61412-ABflo647

Background

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 33-407 of human TrkA (NP_002520.2).
Sequence	AAPCPDACCPHGSSGLRCTRDGALDSLHHLPGAENLTELYIENQQHLQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTCWAENDVGRAEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTEFLEPAANETVRHGCLRLNQPTHVNNGNYTLLAANPFGQASASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSGDP
Gene ID
Swiss Prot
Synonyms	NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; neurotrophic receptor tyrosine kinase 1
Calculated MW	77kDa/83kDa/86kDa/87kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Early endosome membrane, Late endosome membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 647 Rabbit anti-Human TrkA mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Human TrkA mAb (A23974)

Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human TrkA mAb(A23974, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.