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Phospho-TrkA-Y490 Rabbit pAb (AP0492)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH3T3 cells, using Phospho-TrkA-Y490 Rabbit pAb (AP0492).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.

Western blot analysis of lysates from PC-12 cells using Phospho-TrkA-Y490 Rabbit pAb (AP0492) at 1:1800 dilution. PC-12 cells were treated by MNGF (200 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 90s.

Overview

Product name	Phospho-TrkA-Y490 Rabbit pAb
Catalog No.	AP0492
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y490 of human TrkA (NP_001012331.1).
Sequence	HIIEN
Gene ID
Swiss Prot
Synonyms	MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA; Phospho-TrkA-Y490
Calculated MW	87kDa
Observed MW	150kDa/140kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3+EGF, PC-12
Cellular location	Cell membrane, Early endosome membrane, Late endosome membrane, Single-pass type I membrane protein
Customer validation	WB(Homo sapiens)