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ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS076)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS076)

Confocal imaging of HeLa cells using TriMethyl-Histone H3-K9 Rabbit mAb (A22295, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:100)(Red), ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(AS076, dilution 1:200) (Green)

ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS076)

Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (AS076) at 1:100.

Overview

Product nameABflo® 488-conjugated Goat anti-Mouse IgG (H+L)
Catalog No.AS076
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:100 - 1:800
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Homo sapiens, Mus musculus, Drosophila melanogaster)

Documents

Certificate of Compliance

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    ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS076)}

    Immunofluorescence - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS076)

    Confocal imaging of HeLa cells using TriMethyl-Histone H3-K9 Rabbit mAb (A22295, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:100)(Red), ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L)(AS076, dilution 1:200) (Green)
    ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS076)}

    Flow CytoMetry - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS076)

    Flow cytometric analysis of Positive antibody (AE005) (2.5μg/mL) in various cells (orange) compare to Rabbit isotype control (blue) and non-staining control (Red).The secondary antibody used was ABflo® 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (AS076) at 1:100.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS076? Please let us know so that we can cite the reference in this datasheet.