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Caspase-1 Rat mAb (A21085)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Caspase-1 Rat mAb (A21085)

Western blot analysis of various lysates using Caspase-1 Rat mAb (A21085) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rat IgG (H+L) (AS028) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameCaspase-1 Rat mAb
Catalog No.A21085
Host speciesRat
Purification methodAffinity purification
IsotypeIgG
CloneNo.ATC0001
Enables cysteine-type endopeptidase activity. Involved in several processes, including positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-1 beta production; and protein autoprocessing. Acts upstream of or within several processes, including membrane hyperpolarization; mitochondrial depolarization; and positive regulation of interleukin-1 alpha production. Located in extracellular region. Part of IPAF inflammasome complex. Is expressed in knee joint and metanephros. Orthologous to human CASP1 (caspase 1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 206-220aa of mouse Caspase-1.
SequenceTALEMVKEVKEFAAC
Gene ID
Swiss Prot
SynonymsICE; Il1bc
Calculated MW46kDa
Observed MW48kDa/33kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung, Mouse spleen, Rat lung
Cellular locationCytoplasm.
Customer validation

WB(Rattus norvegicus, Mus musculus)

Documents

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    ABclonal:Western blot - Caspase-1 Rat mAb (A21085)}

    Western blot - Caspase-1 Rat mAb (A21085)

    Western blot analysis of various lysates using Caspase-1 Rat mAb (A21085) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rat IgG (H+L) (AS028) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A21085? Please let us know so that we can cite the reference in this datasheet.