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Goat Anti-Mouse IgG (H+L) (AS071)

Publications (12) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - Goat Anti-Mouse IgG (H+L) (AS071)

Western blot analysis of lysates from Mouse IgG using Goat Anti-Mouse IgG (H+L) (AS071) at 1:1000 dilution.
Lysates/proteins: 200ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Goat Anti-Mouse IgG (H+L) (AS071)

Western blot analysis of recombinant Mouse/Rat control IgG Protein using Goat Anti-Mouse IgG (H+L) (AS071) at 1:1000 dilution.
Secondary antibody:HRP Donkey Anti-Goat IgG (H+L)(AS031) at 1:5000 dilution.
Lysates/proteins: 10 ng/5ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameGoat Anti-Mouse IgG (H+L)
Catalog No.AS071
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG.The antibody may cross-react with immunoglobulins from other species.
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa(Light chain), 55kDa(Heavy chain)//25kDa(Light chain), 55kDa(Heavy chain)
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • CUT&Tag 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse IgG, Mouse control IgG
Cellular location
Customer validation

WB(Cavia porcellus, Homo sapiens, Mus musculus, Mus musculus)

FC(Mus musculus)

Co-IP(Homo sapiens)

CUT&Tag(Triticum aestivum)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Goat Anti-Mouse IgG (H+L) (AS071)}

    Western blot - Goat Anti-Mouse IgG (H+L) (AS071)

    Western blot analysis of lysates from Mouse IgG using Goat Anti-Mouse IgG (H+L) (AS071) at 1:1000 dilution.
    Lysates/proteins: 200ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - Goat Anti-Mouse IgG (H+L) (AS071)}

    Western blot - Goat Anti-Mouse IgG (H+L) (AS071)

    Western blot analysis of recombinant Mouse/Rat control IgG Protein using Goat Anti-Mouse IgG (H+L) (AS071) at 1:1000 dilution.
    Secondary antibody:HRP Donkey Anti-Goat IgG (H+L)(AS031) at 1:5000 dilution.
    Lysates/proteins: 10 ng/5ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS071? Please let us know so that we can cite the reference in this datasheet.