제품 > 항체 > 단일클론항체(mAb)

MRP1/ABCC1 Rabbit mAb (A2223)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from A-549 cells, using MRP1/ABCC1 Rabbit mAb (A2223) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1S.

Western blot analysis of various lysates using MRP1/ABCC1 Rabbit mAb (A2223) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MRP1/ABCC1 Rabbit mAb (A2223)

Immunohistochemistry analysis of paraffin-embedded Human colon using MRP1/ABCC1 Rabbit mAb (A2223) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MRP1/ABCC1 Rabbit mAb (A2223) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	MRP1/ABCC1 Rabbit mAb
Catalog No.	A2223
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC52207

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 210-304 of human MRP1/ABCC1 (NP_004987.2).
Sequence	ESSASFLSRITFWWITGLIVRGYRQPLEGSDLWSLNKEDTSEQVVPVLVKNWKKECAKTRKQPVKVVYSSKDPAQPKESSKVDANEEVEALIVKS
Gene ID
Swiss Prot
Synonyms	MRP; ABCC; GS-X; MRP1; ABC29; DFNA77; MRP1/ABCC1
Calculated MW	172kDa
Observed MW	170-220kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	A549, Mouse lung, Rat skeletal muscle, Rat heart
Cellular location	apical plasma membrane, basal plasma membrane, basolateral plasma membrane, extracellular exosome, lateral plasma membrane, plasma membrane