제품 > 항체 > 다중클론항체(pAb)

Occludin Rabbit pAb (A12621)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of 293T, using Occludin antibody (A12621) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Review

Overview

Product name	Occludin Rabbit pAb
Catalog No.	A12621
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 350-450 of human Occludin (NP_002529.1).
Sequence	VVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSEL
Gene ID
Swiss Prot
Synonyms	BLCPMG; PTORCH1; PPP1R115
Calculated MW	59kDa
Observed MW	62kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T
Cellular location	Cell junction, Membrane, Multi-pass membrane protein, tight junction
Customer validation	(HK2) WB(Rattus norvegicus, Mus musculus, Homo sapiens, Sus scrofa, Other, Gallus gallus) IF(Sus scrofa)

Documents

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Research Area

연구세포 생물학 & 발달 생물학세포 접착밀착연접

Occludin Rabbit pAb images

Western blot - Occludin Rabbit pAb (A12621)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Front Psychiatry

Application:

IF:

4.157

Species:

Rattus norvegicus

PMID:

33033482

Title:

Supplementation of Diet With Different n-3/n-6 PUFA Ratios Ameliorates Autistic Behavior, Reduces Serotonin, and Improves Intestinal Barrier Impairments in a Valproic Acid Rat Model of Autism

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

36421064

Title:

Microalgae oil from Schizochytrium sp. alleviates obesity and modulates gut microbiota in high-fat diet-fed mice

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

American journal of physiology. Gastrointestinal and liver physiology

Application:

IF:

4.87

Species:

Homo sapiens

PMID:

35638642

Title:

Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases (IBD) by suppressing autophagy activation in mice

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Homo sapiens

PMID:

37180168

Title:

CD73 mediated host purinergic metabolism in intestine contributes to the therapeutic efficacy of a novel mesenchymal-like endometrial regenerative cells against experimental colitis - PMC

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Scientific reports

Application:

IF:

4.99

Species:

Mus musculus

PMID:

37095192

Title:

Gut microbiota induces hepatic steatosis by modulating the T cells balance in high fructose diet mice - PMC

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Current research in food science

Application:

IF:

6.26

Species:

Mus musculus

PMID:

37351541

Title:

Lactoferrin alleviates Western diet-induced cognitive impairment through the microbiome-gut-brain axis - PMC

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Mus musculus

PMID:

37446646

Title:

Polysaccharides from Paecilomyces hepiali Prevent Acute Colitis in Association with Modulating Gut Microbiota and Treg/Th17 Immune Balance in Mice - PMC

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Veterinary microbiology

Application:

IF:

3.24

Species:

Sus scrofa

PMID:

37068404

Title:

PRRSV-induced inflammation in pulmonary intravascular macrophages (PIMs) and pulmonary alveolar macrophages (PAMs) contributes to endothelial barrier …

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Brain and behavior

Application:

IF:

Species:

Rattus norvegicus

PMID:

37596045

Title:

Prangos ferulacea (L.) ameliorates behavioral alterations, hippocampal oxidative stress markers, and apoptotic deficits in a rat model of autism induced by valproic

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

Application:

IF:

4.52

Species:

Homo sapiens

PMID:

37611884

Title:

Melatonin reduced colon inflammation but had no effect on energy metabolism in ageing Mongolian gerbils (Meriones unguiculatus)

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Toxicology

Application:

IF:

4.57

Species:

Homo sapiens

PMID:

37442268

Title:

Selenium nanoparticles alleviate deoxynivalenol-induced intestinal epithelial barrier dysfunction by regulating endoplasmic reticulum stress in IPEC-J2 cells

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Food & function

Application:

IF:

6.31

Species:

Mus musculus

PMID:

37853813

Title:

Pectic oligosaccharides ameliorate high-fat diet-induced obesity and hepatic steatosis in association with modulating gut microbiota in mice

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Frontiers in cellular and infection microbiology

Application:

IF:

5.7

Species:

Other

PMID:

38390621

Title:

Microbial interventions in yak colibacillosis: Lactobacillus-mediated regulation of intestinal barrier - PMC

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

WB, IF

IF:

8.02

Species:

Sus scrofa

PMID:

36565836

Title:

Sodium butyrate protects against rotavirus-induced intestinal epithelial barrier damage by activating AMPK-Nrf2 signaling pathway in IPEC-J2 cells

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Human reproduction (Oxford, England)

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

38783610

Title:

Excessive proliferating cell nuclear antigen attenuates endometrial adhesive capacity and decidualization in patients with recurrent implantation failure

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Frontiers in cellular and infection microbiology

Application:

IF:

5.7

Species:

Gallus gallus

PMID:

35252040

Title:

Probiotic Bacillus subtilis LF11 Protects Intestinal Epithelium Against Salmonella Infection

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Food & function

Application:

IF:

6.1

Species:

Other

PMID:

38469921

Title:

Exopolysaccharide production by Lactobacillus plantarum T10 is responsible for the probiotic activity in enhancing intestinal barrier function in vitro and in vivo

References for Occludin Rabbit pAb (A12621)

Product:

Occludin Rabbit pAb

Journal:

Animal nutrition (Zhongguo xu mu shou yi xue hui)

Application:

IF:

6.1

Species:

Other

PMID:

39315328