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HRP-conjugated Goat anti-Mouse IgG2a (AS065)

Publications (19) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP-conjugated Goat anti-Mouse IgG2a (AS065)

Western blot analysis of lysates from Mouse IgG using HRP Goat Anti-Mouse IgG2a (AS065) at 1:2000 dilution.
Lysates/proteins: 100ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameHRP-conjugated Goat anti-Mouse IgG2a
Catalog No.AS065
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG2a
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa(Light chain), 55kDa(Heavy chain)
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:5000
  • ELISA 1:2000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50% glycerol, pH7.4.
Key applicationWestern blotting    
Positive samplesMouse IgG2a
Cellular location
Customer validation

WB(Mus musculus, Mesocricetus auratus, Sus scrofa, Homo sapiens, Mus musculus, Bos taurus)

ELISA(Mus musculus, Capra hircus, Sus scrofa, Toxoplasma gondii, Homo sapiens)

IP(Mus musculus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - HRP-conjugated Goat anti-Mouse IgG2a (AS065)}

    Western blot - HRP-conjugated Goat anti-Mouse IgG2a (AS065)

    Western blot analysis of lysates from Mouse IgG using HRP Goat Anti-Mouse IgG2a (AS065) at 1:2000 dilution.
    Lysates/proteins: 100ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS065? Please let us know so that we can cite the reference in this datasheet.