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MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Western blot analysis of various lysates using MonoMethyl-Histone H3-K27 Rabbit pAb (A2361) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Dot Blot - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody (A2361).

ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using MonoMethyl-Histone H3-K27 Rabbit pAb (A2361) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MonoMethyl-Histone H3-K27 Rabbit pAb (A2361) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using MonoMethyl-Histone H3-K27 Rabbit pAb (A2361) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K27 Rabbit pAb (A2361) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using MonoMethyl-Histone H3-K27 Rabbit pAb (A2361) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K27 antibody (A2361) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review

Overview

Product name	MonoMethyl-Histone H3-K27 Rabbit pAb
Catalog No.	A2361
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic monomethylated peptide around K27 of human histone H3 (NP_003520.1).
Sequence	ARKSA
Gene ID
Swiss Prot
Synonyms	H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; MonoMethyl-Histone H3-K27
Calculated MW	15kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 DB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa acid extract, NIH/3T3 acid extract, H3 protein
Cellular location	Chromosome, Nucleus.
Customer validation	(293FT,PC9) WB(Solanum lycopersicum, Mus musculus, Musa nana Lour, Eurosta solidaginis, Homo sapiens, Other) IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

MonoMethyl-Histone H3-K27 Rabbit pAb images

Western blot - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Dot Blot - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K27 antibody (A2361).

Immunohistochemistry - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Immunofluorescence - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2361

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Reviews List

Product Protocols

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

The New Phytologist

Application:

IF:

10.151

Species:

Solanum lycopersicum

PMID:

32255501

Title:

Histone demethylase SlJMJ6 promotes fruit ripening by removing H3K27 methylation of ripening-related genes in tomato

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

Journal of microbiology (Seoul, Korea)

Application:

IF:

2.9

Species:

Mus musculus

PMID:

35437622

Title:

Gamma-glutamyltransferase of Helicobacter pylori alters the proliferation, migration, and pluripotency of mesenchymal stem cells by affecting metabolism and methylation status

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

Food chemistry

Application:

IF:

9.23

Species:

Musa nana Lour

PMID:

36495744

Title:

Histone demethylase MaJMJ15 is involved in the regulation of postharvest banana fruit ripening

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Eurosta solidaginis

PMID:

37373302

Title:

Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall Fly Eurosta solidaginis - PMC

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

IF:

5.486

Species:

Homo sapiens

PMID:

35278430

Title:

Downregulation of the endothelial histone demethylase JMJD3 is associated with neointimal hyperplasia of arteriovenous fistulas in kidney failure

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

Stem cell research & therapy

Application:

WB, IF

IF:

8.07

Species:

Mus musculus

PMID:

35715822

Title:

Glutamate regulates gliosis of BMSCs to promote ENS regeneration through -KG and H3K9/H3K27 demethylation - PMC

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

Plant physiology

Application:

IF:

7.4

Species:

Other

PMID:

38669310

Title:

Histone H3K27 Demethylase SlJMJ3 Modulates Fruit Ripening in Tomato

References for MonoMethyl-Histone H3-K27 Rabbit pAb (A2361)

Product:

MonoMethyl-Histone H3-K27 Rabbit pAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Mus musculus

PMID:

39148040

Title:

Gamma-glutamyl transferase secreted by Helicobacter pylori promotes the development of gastric cancer by affecting the energy metabolism and histone methylation status of gastric epithelial cells - PMC