Reviews (3)Publications (40) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Western blot analysis of various lysates using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K4 antibody (A2355).
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse stomach using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using MonoMethyl-Histone H3-K4 Rabbit pAb (A2355) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K4 Rabbit pAb antibody (A2355) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K4 antibody (A2355) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| Product name | MonoMethyl-Histone H3-K4 Rabbit pAb |
|---|---|
| Catalog No. | A2355 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic monomethylated peptide around K4 of human histone H3 (NP_003520.1). |
|---|---|
| Sequence | RTKQT |
| Gene ID | |
| Swiss Prot | |
| Synonyms | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; MonoMethyl-Histone H3-K4 |
| Calculated MW | 16kDa |
| Observed MW | 17kDa |
| Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HeLa, NIH/3T3, C6, H3 |
| Cellular location | Chromosome, Nucleus. |
| Customer validation | (Prawn gills, Human cells, HeLa) WB(Mus musculus, Homo sapiens, Solanum lycopersicum, Musa nana Lour, Danio rerio, Oryza sativa, Saccharomyces cerevisiae, Other) ChIP-Seq(Homo sapiens, Zea mays, Oryza sativa, Mus musculus) IF(Homo sapiens) IHC(Homo sapiens) CUT&Tag(Other) ChIP(Oryza sativa, Mus musculus, Other, Arabidopsis thaliana, Homo sapiens) Co-IP(Mus musculus) |
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(Me)_WB_01.jpg?t=1721357762 "ABclonal:Western blot - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
(2)_DB_01.jpg?t=1721357762 "ABclonal:Dot Blot - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
_IF_01.jpg?t=1721357762 "ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
_IF_01.jpg?t=1721357762 "ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
_IF_02.jpg?t=1721357762 "ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
_IF_03.jpg?t=1721357762 "ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
_IF_01.jpg?t=1721357762 "ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
_IF_02.jpg?t=1721357762 "ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
(2)_ChIP_01.jpg?t=1721357762 "ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
_ChIP_01.jpg?t=1721357762 "ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K4 Rabbit pAb (A2355)")
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