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Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from HeLa, NIH/3T3, C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at 1:1000 dilution. HeLa, NIH/3T3 and C6 cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Confocal imaging of NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of HeLa cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal imaging of C6 cells using Phospho-Histone H3-S10 Rabbit mAb (AP0002, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunohistochemistry - Phospho-Histone H3-S10 Rabbit mAb (AP0002)

Immunohistochemistry analysis of paraffin-embedded Human oophoroma using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.05M Tris/EDTA Buffer (pH 8.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-Histone H3-S10 Rabbit mAb (AP0002) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.05M Tris/EDTA Buffer (pH 8.0) prior to IHC staining.

Review

Overview

Product name	Phospho-Histone H3-S10 Rabbit mAb
Catalog No.	AP0002
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0003

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S10 of human Histone H3 (P68431).
Sequence	ARKST
Gene ID
Swiss Prot
Synonyms	H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Phospho-Histone H3-S10
Calculated MW	15kDa
Observed MW	15kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa treated by nocodazole, NIH/3T3 treated by nocodazole, C6 treated by nocodazole
Cellular location	Chromosome, Nucleus
Customer validation	(HEK293T cells) WB(Homo sapiens) IF(Homo sapiens, Mus musculus)