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Lactic acid-Histone H3-K18 Rabbit pAb (A18807)

Publications (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Lactic acid-Histone H3-K18 Rabbit pAb (A18807)

Western blot analysis of lysates from MCF7 cells, using Lactic acid-Histone H3-K18 Rabbit pAb (A18807) at 1:500 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.

ABclonal:Dot Blot - Lactic acid-Histone H3-K18 Rabbit pAb (A18807)

Dot-blot analysis of all sorts of peptides using Lactic acid-Histone H3-K18 antibody (A18807) at 1:1000 dilution.

Overview

Product nameLactic acid-Histone H3-K18 Rabbit pAb
Catalog No.A18807
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic lactylated peptide around K18 of human Histone H3 (NP_003520.1).
SequenceAPRKQ
Gene ID
Swiss Prot
SynonymsH3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Lactic acid-Histone H3-K18
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • DB 1:500 - 1:4000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7 treated by Sodium lactate
Cellular locationChromosome, Nucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - Lactic acid-Histone H3-K18 Rabbit pAb (A18807)}

    Western blot - Lactic acid-Histone H3-K18 Rabbit pAb (A18807)

    Western blot analysis of lysates from MCF7 cells, using Lactic acid-Histone H3-K18 Rabbit pAb (A18807) at 1:500 dilution. MCF7 cells were treated by Sodium lactate(100mM) for 24h.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5min.
    ABclonal:Dot Blot - Lactic acid-Histone H3-K18 Rabbit pAb (A18807)}

    Dot Blot - Lactic acid-Histone H3-K18 Rabbit pAb (A18807)

    Dot-blot analysis of all sorts of peptides using Lactic acid-Histone H3-K18 antibody (A18807) at 1:1000 dilution.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A18807? Please let us know so that we can cite the reference in this datasheet.

    항체 (105)

    Secondary Antibodies (26)