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Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Dot Blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (A7257) at 1:1000 dilution.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunohistochemistry analysis of paraffin-embedded Rat brain using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb antibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Acetyl-Histone H3-K18 Rabbit pAb yantibody (A7257) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name	Acetyl-Histone H3-K18 Rabbit pAb
Catalog No.	A7257
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic acetylated peptide around K18 of human H3 (NP_003520.1).
Sequence	PRKQL
Gene ID
Swiss Prot
Synonyms	H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J; Acetyl-Histone H3-K18
Calculated MW	15kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 DB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa treated by TSA
Cellular location	Chromosome, Nucleus.
Customer validation	WB(Mus musculus, Homo sapiens, Monascus ruber, Oryza sativa Japonica Group, Eurosta solidaginis, Other) ChIP(Homo sapiens, Other)

Documents

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Research Area

Acetyl-Histone H3-K18 Rabbit pAb images

Western blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Dot Blot - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody (A7257) at 1:1000 dilution.

Immunohistochemistry - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 Rabbit pAb (A7257) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Chromatin Immunoprecipitation - Acetyl-Histone H3-K18 Rabbit pAb (A7257)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

Cancer Cell

Application:

IF:

31.743

Species:

Mus musculus

PMID:

32442403

Title:

SETD5-Coordinated Chromatin Reprogramming Regulates Adaptive Resistance to Targeted Pancreatic Cancer Therapy

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

J Virol

Application:

IF:

5.1

Species:

Homo sapiens

PMID:

32269118

Title:

Histone Deacetylase Inhibitors Promote Latent Adenovirus Reactivation from Tonsillectomy Specimens

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

Microbiol Spectr

Application:

IF:

7.171

Species:

Homo sapiens

PMID:

34668748

Title:

Characterization of Tonsil Microbiota and Their Effect on Adenovirus Reactivation in Tonsillectomy Samples

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

Applied biochemistry and biotechnology

Application:

IF:

3.09

Species:

Monascus ruber

PMID:

35802237

Title:

Inactivation of MrSir2 in Monascus ruber Influenced the Developmental Process and the Production of Monascus Azaphilone Pigments

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

Journal of integrative plant biology

Application:

IF:

9.1

Species:

Oryza sativa Japonica Group

PMID:

36807738

Title:

Histone deacetylase OsHDA706 increases salt tolerance via H4K5/K8 deacetylation of OsPP2C49 in rice

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

Journal of applied microbiology

Application:

IF:

4.05

Species:

Monascus ruber

PMID:

36849138

Title:

Mrhst4 gene, coding for NAD+-dependent deacetylase is involved in citrinin production of Monascus ruber

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Eurosta solidaginis

PMID:

37373302

Title:

Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall Fly Eurosta solidaginis - PMC

References for Acetyl-Histone H3-K18 Rabbit pAb (A7257)

Product:

Acetyl-Histone H3-K18 Rabbit pAb

Journal:

Journal of basic microbiology

Application:

IF:

2.65

Species:

Homo sapiens

PMID:

37236161