제품 > 항체 > 인산화 특이적 항체

Phospho-Histone H3.3-T3 Rabbit mAb (AP1152)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3.3-T3 Rabbit mAb (AP1152) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3.3-T3 Rabbit mAb (AP1152) at 1:1000 dilution. HeLa cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from C6 cells, using Phospho-Histone H3.3-T3 Rabbit mAb (AP1152) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product name	Phospho-Histone H3.3-T3 Rabbit mAb
Catalog No.	AP1152
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1662

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T3 of human Histone H3 (P84243).
Sequence	MARTK
Gene ID
Swiss Prot
Synonyms	H3F3; H3-3B; H3.3A; H3F3A; BRYLIB1; Phospho-Histone H3.3-T3
Calculated MW	15kDa
Observed MW	16kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Nocodazole, NIH/3T3 treated by Nocodazole, C6 treated by Nocodazole
Cellular location	Chromosome, Nucleus.