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MonoMethyl-Histone H3-K4 Rabbit mAb (A22078)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1 μg MonoMethyl-Histone H3-K4 antibody (A22078), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of H3K4me1 in representative gene loci (RPL30), as shown in figure.

ABclonal:Western blot - MonoMethyl-Histone H3-K4 Rabbit mAb (A22078)

Western blot analysis of various lysates using MonoMethyl-Histone H3-K4 Rabbit mAb (A22078) at1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Dot Blot - MonoMethyl-Histone H3-K4 Rabbit mAb (A22078)

Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K4 antibody (A22078) at 1:2000 dilution.

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K4 Rabbit mAb (A22078)

Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K4 Rabbit mAb (A22078) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K4 Rabbit mAb (A22078) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using MonoMethyl-Histone H3-K4 Rabbit mAb (A22078) at dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal imaging of HeLa cells using MonoMethyl-Histone H3-K4 Rabbit mAb (A22078, dilution 1:300) (Green). The cells were counterstained with [KO Validated] NF-kB p65/RelA Rabbit mAb (A22331, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Review

Overview

Product name	MonoMethyl-Histone H3-K4 Rabbit mAb
Catalog No.	A22078
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC54646

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic monomethylated peptide around K4 of human Histone H3 (NP_003520.1).
Sequence	MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Gene ID
Swiss Prot
Synonyms	H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; MonoMethyl-Histone H3-K4
Calculated MW	16kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:20000 DB 1:1000 - 1:5000 IF/ICC 1:100 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. CUT&Tag 10⁵ cells /1 μg
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, NIH/3T3, C6
Cellular location	Chromosome, Nucleus.
Customer validation	(Tilapia mossambica) WB(Mus musculus)