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TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from HeLa cells, using TriMethyl-Histone H3-K79 Rabbit pAb (A2369) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.

ABclonal:Dot Blot - TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody (A2369) at 1:1000 dilution.

ABclonal:Chromatin Immunoprecipitation - TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TriMethyl-Histone H3-K79 antibody (A2369) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review

Overview

Product name	TriMethyl-Histone H3-K79 Rabbit pAb
Catalog No.	A2369
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic trimethylated peptide around K79 of human histone H3 (NP_003520.1).
Sequence	DFKTD
Gene ID
Swiss Prot
Synonyms	H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K79
Calculated MW	16kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 DB 1:500 - 1:2000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa
Cellular location	Chromosome, Nucleus.
Customer validation	(HCT116, HEK293T, Mouse testis, HeLa) WB(Mus musculus, Homo sapiens, Eurosta solidaginis, Saccharomyces cerevisiae) ChIP(Saccharomyces cerevisiae)

Documents

Certificate of Compliance

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Lot number

Research Area

TriMethyl-Histone H3-K79 Rabbit pAb images

Western blot - TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Dot Blot - TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K79 antibody (A2369) at 1:1000 dilution.

Chromatin Immunoprecipitation - TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

* For research use only. Not for therapeutic or diagnostic purposes.

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Product Protocols

References for TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Product:

TriMethyl-Histone H3-K79 Rabbit pAb

Journal:

J Inflamm Res

Application:

IF:

6.922

Species:

Mus musculus

PMID:

34824542

Title:

Aberrant Fluid Shear Stress Contributes to Articular Cartilage Pathogenesis via Epigenetic Regulation of ZBTB20 by H3K4me3

References for TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Product:

TriMethyl-Histone H3-K79 Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

IF:

Species:

Homo sapiens

PMID:

35690146

Title:

Histone methyltransferase Dot1L recruits O-GlcNAc transferase to target chromatin sites to regulate histone O-GlcNAcylation - PMC

References for TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Product:

TriMethyl-Histone H3-K79 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Eurosta solidaginis

PMID:

37373302

Title:

Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall Fly Eurosta solidaginis - PMC

References for TriMethyl-Histone H3-K79 Rabbit pAb (A2369)

Product:

TriMethyl-Histone H3-K79 Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Saccharomyces cerevisiae

PMID:

36167807