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Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from C6 cells using Acetyl-Histone H3-K14 Rabbit pAb (A7254) at 1:400 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from NIH/3T3 cells using Acetyl-Histone H3-K14 Rabbit pAb (A7254) at 1:400 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from HeLa cells using Acetyl-Histone H3-K14 Rabbit pAb (A7254) at 1:400 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K14 Rabbit pAb (A7254) at dilution of 1:100 (40x lens). U-2 OS cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Acetyl-Histone H3-K14 Rabbit pAb
Catalog No.	A7254
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic acetylated peptide around K14 of human H3 (NP_003520.1).
Sequence	GGKAP
Gene ID
Swiss Prot
Synonyms	H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K14
Calculated MW	16kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	C6 treated by TSA, NIH/3T3 treated by TSA, HeLa treated by TSA
Cellular location	Chromosome, Nucleus.
Customer validation	(HL-60) WB(Rattus norvegicus, Mus musculus, Monascus ruber, A. fumigatus, Eurosta solidaginis, Homo sapiens, Mus musculus, Other, Danio rerio) IP(Rattus norvegicus) ChIP(Homo sapiens, Rattus norvegicus, Mus musculus) Co-IP(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Acetyl-Histone H3-K14 Rabbit pAb images

Western blot - Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Immunofluorescence - Acetyl-Histone H3-K14 Rabbit pAb (A7254)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A7254

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Reviews List

Product Protocols

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Epigenetics

Application:

IF:

4.528

Species:

Rattus norvegicus

PMID:

30821590

Title:

Corticosterone rather than ethanol epigenetic programmed testicular dysplasia caused by prenatal ethanol exposure in male offspring rats.

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Cell Biol Toxicol

Application:

IF:

6.691

Species:

Rattus norvegicus

PMID:

33619658

Title:

Programming changes of hippocampal miR-134-5p/SOX2 signal mediate the susceptibility to depression in prenatal dexamethasone-exposed female offspring

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Cancer Cell

Application:

IF:

31.743

Species:

Mus musculus

PMID:

32442403

Title:

SETD5-Coordinated Chromatin Reprogramming Regulates Adaptive Resistance to Targeted Pancreatic Cancer Therapy

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Applied biochemistry and biotechnology

Application:

IF:

3.09

Species:

Monascus ruber

PMID:

35802237

Title:

Inactivation of MrSir2 in Monascus ruber Influenced the Developmental Process and the Production of Monascus Azaphilone Pigments

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Cell reports

Application:

IF:

Species:

Mus musculus

PMID:

36450263

Title:

β-hydroxybutyrate reduces reinstatement of cocaine conditioned place preference through hippocampal CaMKII-α β-hydroxybutyrylation

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

7.46

Species:

A. fumigatus

PMID:

36374932

Title:

Aspergillus fumigatus Elongator complex subunit 3 affects hyphal growth, adhesion and virulence through wobble uridine tRNA modification - PMC

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Eurosta solidaginis

PMID:

37373302

Title:

Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall Fly Eurosta solidaginis - PMC

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Acta pharmaceutica Sinica. B

Application:

IF:

14.903

Species:

Homo sapiens, Mus musculus

PMID:

33777677

Title:

A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

The Journal of endocrinology

Application:

ChIP

IF:

4.66

Species:

Homo sapiens

PMID:

31141788

Title:

The GC-IGF1 axis-mediated testicular dysplasia caused by prenatal caffeine exposure

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens, Mus musculus

PMID:

37260741

Title:

Elevated expression of histone deacetylase HDAC8 suppresses arginine-proline metabolism in necrotizing enterocolitis - PMC

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Food & function

Application:

ChIP

IF:

6.31

Species:

Homo sapiens

PMID:

38113052

Title:

The high-expression programming of SR-B1 mediates adrenal dysfunction in female offspring induced by prenatal caffeine exposure and its cholesterol accumulation

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Applied and environmental microbiology

Application:

IF:

4.4

Species:

Other

PMID:

38451077

Title:

Histone acetyltransferase Sas3 contributes to fungal development, cell wall integrity, and virulence in Aspergillus fumigatus

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

FASEB Journal

Application:

ChIP

IF:

5.834

Species:

Homo sapiens

PMID:

29718709

Title:

Intrauterine programming mechanism for hypercholesterolemia in prenatal caffeineexposed female adult rat offspring

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Toxicology letters

Application:

ChIP

IF:

4.271

Species:

Rattus norvegicus

PMID:

31306741

Title:

Decreased H3K9ac level of KLF4 mediates podocyte developmental toxicity induced by prenatal caffeine exposure in male offspring rats.

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Toxicology and applied pharmacology

Application:

ChIP

IF:

4.46

Species:

Rattus norvegicus

PMID:

31075344

Title:

Glucocorticoid programming mechanism for hypercholesterolemia in prenatal ethanol-exposed adult offspring rats.

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids

Application:

ChIP

IF:

5.228

Species:

Rattus norvegicus

PMID:

31513924

Title:

Decreased H3K9 acetylation level of LXR mediated dexamethasone-induced placental cholesterol transport dysfunction.

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Toxicol Appl Pharmacol

Application:

ChIP

IF:

4.46

Species:

Mus musculus

PMID:

32791177

Title:

Epigenetic repression of AT2 receptor is involved in cell dysfunction and glucose intolerance of adult female offspring rats exposed to dexamethasone prenatally

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

British journal of pharmacology

Application:

ChIP

IF:

9.473

Species:

Rattus norvegicus

PMID:

32757222

Title:

The lowexpression programming of 11HSD2 mediates osteoporosis susceptibility induced by prenatal caffeine exposure in male offspring rats

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Toxicology

Application:

ChIP

IF:

4.571

Species:

Rattus norvegicus

PMID:

29548890

Title:

The Expressional Disorder of the Renal RAS Mediates Nephrotic Syndrome of Male Rat Offspring Induced by Prenatal Ethanol Exposure

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

The American Journal of Pathology

Application:

ChIP

IF:

5.77

Species:

Rattus norvegicus

PMID:

30273601

Title:

Intrauterine programming of Glucocorticoid-Insulin-Like Growth Factor 1 axis-mediated developmental origin of osteoporosis susceptibility in female offspring rats of prenatal caffeine exposure.

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Toxicology

Application:

ChIP

IF:

4.571

Species:

Rattus norvegicus

PMID:

30825513

Title:

Caffeine programs hepatic SIRT1-related cholesterol synthesis and hypercholesterolemia via A2AR/cAMP/PKA pathway in adult male offspring rats.

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Toxicological sciences : an official journal of the Society of Toxicology

Application:

Co-IP

IF:

4.84

Species:

Rattus norvegicus

PMID:

31518422

Title:

Prenatal dexamethasone exposure induced alterations in neurobehavior and hippocampal glutamatergic system balance in female rat offspring

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Toxicological sciences : an official journal of the Society of Toxicology

Application:

ChIP

IF:

4.1

Species:

Rattus norvegicus

PMID:

36790081

Title:

Epigenetic programming of TBX2/CX43 mediates lower sperm quality in male offspring induced by prenatal dexamethasone exposure

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

ChIP

IF:

5.83

Species:

Rattus norvegicus

PMID:

37249374

Title:

Autophagy inhibition mediated by intrauterine miR19123p/CTSD programming participated in the susceptibility to osteoarthritis induced by prenatal dexamethasone

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Biochemical pharmacology

Application:

ChIP

IF:

6.1

Species:

Rattus norvegicus

PMID:

36174767

Title:

Positive programming of the GC-IGF1 axis mediates adult osteoporosis susceptibility in male offspring rats induced by prenatal dexamethasone exposure

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

8.9

Species:

Danio rerio

PMID:

38588970

Title:

Early life stage exposure to fenbuconazole causes multigenerational cardiac developmental defects in zebrafish and potential reasons

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Pharmacological research

Application:

ChIP

IF:

10.33

Species:

Rattus norvegicus

PMID:

34656764

Title:

Sex difference in adrenal developmental toxicity induced by dexamethasone and its intrauterine programming mechanism

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Chinese medicine

Application:

IF:

4.9

Species:

Rattus norvegicus

PMID:

38956695

Title:

Angelicin improves osteoporosis in ovariectomized rats by reducing ROS production in osteoclasts through regulation of the KAT6A/Nrf2 signalling pathway - PMC

References for Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Product:

Acetyl-Histone H3-K14 Rabbit pAb

Journal:

Cell reports. Medicine

Application:

IF:

14.3

Species:

Homo sapiens

PMID:

38301654