제품 > 항체 > 메틸화 특이적 항체

DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from HeLa cells, using DiMethyl-Histone H3-K36 Rabbit pAb (A2365) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of various lysates, using DiMethyl-Histone H3-K36 Rabbit pAb (A2365) at 1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Dot Blot - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (A2365).

ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Immunohistochemistry analysis of paraffin-embedded Human breast using DiMethyl-Histone H3-K36 Rabbit pAb (A2365) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis using DiMethyl-Histone H3-K36 Rabbit pAb (A2365) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using DiMethyl-Histone H3-K36 Rabbit pAb (A2365) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 Rabbit pAb (A2365). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K36 antibody (A2365) and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review

Overview

Product name	DiMethyl-Histone H3-K36 Rabbit pAb
Catalog No.	A2365
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic dimethylated peptide around K36 of human histone H3 (NP_003520.1).
Sequence	GVKKP
Gene ID
Swiss Prot
Synonyms	H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; DiMethyl-Histone H3-K36
Calculated MW	16kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 5μg-10μg of Chromatin ChIP-seq 1:20 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, NIH/3T3, C6, H3
Cellular location	Chromosome, Nucleus.
Customer validation	(Hippocampus, HeLa) WB(Homo sapiens, Penicillium oxalicum, Oryza sativa, Mus musculus, Saccharomyces cerevisiae) ChIP(Mus musculus) IHC(Mus musculus) WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

DiMethyl-Histone H3-K36 Rabbit pAb images

Western blot - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Dot Blot - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (A2365).

Immunohistochemistry - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Immunofluorescence - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 Rabbit pAb (A2365). Blue: DAPI for nuclear staining.

Chromatin Immunoprecipitation - DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2365

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Product:

DiMethyl-Histone H3-K36 Rabbit pAb

Journal:

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine

Application:

IF:

2.949

Species:

Homo sapiens

PMID:

29951879

Title:

Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer.

References for DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Product:

DiMethyl-Histone H3-K36 Rabbit pAb

Journal:

Cell Discov

Application:

IF:

10.84

Species:

Homo sapiens

PMID:

33972509

Title:

Cryo-EM structure of SETD2/Set2 methyltransferase bound to a nucleosome containing oncohistone mutations

References for DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Product:

DiMethyl-Histone H3-K36 Rabbit pAb

Journal:

Biotechnol Biofuels

Application:

IF:

6.04

Species:

Penicillium oxalicum

PMID:

34952627

Title:

Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1–Cyc8 complex in Penicillium oxalicum and Trichoderma reesei

References for DiMethyl-Histone H3-K36 Rabbit pAb (A2365)

Product:

DiMethyl-Histone H3-K36 Rabbit pAb

Journal:

J Integr Plant Biol

Application:

IF:

9.106

Species:

Oryza sativa

PMID:

34845826