Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse
Western blot analysis of lysates from HT-29 cells, using Phospho-RIPK1/RIP-S166 Rabbit pAb (AP1230) at 1:800 dilution. HT-29 cells were treated with Z-VAD (50uM), human TNF-α (50ng/ml) and SM-164 (200nM) at 37℃ for 5 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
| Product name | Phospho-RIPK1/RIP-S166 Rabbit pAb |
|---|---|
| Catalog No. | AP1230 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic phosphorylated peptide around S166 of human RIPK1/RIP (NP_003795.2). |
|---|---|
| Sequence | MWSKL |
| Gene ID | |
| Swiss Prot | |
| Synonyms | RIP; RIP1; AIEFL; IMD57; RIP-1; Phospho-RIPK1/RIP-S166 |
| Calculated MW | 76kDa |
| Observed MW | 75kDa |
| Reactivity | Human, Mouse |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HT-29 treated with Z-VAD, TNF-α and SM-164 |
| Cellular location | cytosol, mitochondrion, plasma membrane, ripoptosome. |
| Customer validation | WB(Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Mus musculus) IF(Mus musculus) ELISA(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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