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TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of various lysates, using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Dot Blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody (A2372).

ABclonal:Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunohistochemistry analysis of paraffin-embedded Rat testis using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit pAb (A2372). Blue: DAPI for nuclear staining.

Review

Overview

Product name	TriMethyl-Histone H4-K20 Rabbit pAb
Catalog No.	A2372
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

Immunogen	A synthetic trimethylated peptide around K20 of human histone H4 (NP_003529.1).
Sequence	HRKVL
Gene ID
Swiss Prot
Synonyms	H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; TriMethyl-Histone H4-K20
Calculated MW	11kDa
Observed MW	11kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 DB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, NIH/3T3, C6, H4
Cellular location	Chromosome, Nucleus.
Customer validation	(Mouse uterus) WB(Mus musculus, Eurosta solidaginis, Homo sapiens) IF(Capra hircus, Mus musculus)

Documents

Certificate of Compliance

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Research Area

연구단백질 인산화

TriMethyl-Histone H4-K20 Rabbit pAb images

Western blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Dot Blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody (A2372).

Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunofluorescence - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit pAb (A2372). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Product:

TriMethyl-Histone H4-K20 Rabbit pAb

Journal:

Clinical epigenetics

Application:

IF:

6.551

Species:

Mus musculus

PMID:

30867030

Title:

The hyper-activation of transcriptional enhancers in breast cancer.

References for TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Product:

TriMethyl-Histone H4-K20 Rabbit pAb

Journal:

American journal of translational research

Application:

IF:

3.94

Species:

Capra hircus

PMID:

36105059

Title:

Epigenetic dynamics of H4K20me3 modification during oocyte maturation and early reprogramming of somatic cell nuclear transfer goat embryos - PMC

References for TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Product:

TriMethyl-Histone H4-K20 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Eurosta solidaginis

PMID:

37373302

Title:

Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall Fly Eurosta solidaginis - PMC

References for TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Product:

TriMethyl-Histone H4-K20 Rabbit pAb

Journal:

Molecular Cell

Application:

IF:

19.328

Species:

Mus musculus

PMID:

31677973