제품 > 항체 > 다중클론항체(pAb)

Histone H4 Rabbit pAb (A1131)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of various lysates, using Histone H4 Rabbit pAb (A1131) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review

Overview

Product name	Histone H4 Rabbit pAb
Catalog No.	A1131
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_003529.1).
Sequence	MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYG
Gene ID
Swiss Prot
Synonyms	H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4A; Histone H4
Calculated MW	11kDa
Observed MW	11kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa, HepG2, Raji, MCF-7, NIH3T3, Mouse Thymus
Cellular location	extracellular exosome, extracellular region, nucleoplasm, nucleus.
Customer validation	(U87MG) WB(Mus musculus, Homo sapiens, Saccharomyces cerevisiae, Arabidopsis thaliana, Mus musculus, Rattus norvegicus, Nicotiana benthamiana) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구단백질 인산화

Histone H4 Rabbit pAb images

Western blot - Histone H4 Rabbit pAb (A1131)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Histone H4 Rabbit pAb (A1131)

Product:

Histone H4 Rabbit pAb

Journal:

Genome Biology

Application:

IF:

13.583

Species:

Mus musculus

PMID:

32085783

Title:

Histone H3K27 acetylation is dispensable for enhancer activity in mouse embryonic stem cells

References for Histone H4 Rabbit pAb (A1131)

Product:

Histone H4 Rabbit pAb

Journal:

American Journal of Translational Research

Application:

IF:

4.06

Species:

Mus musculus

PMID:

27829992

Title:

HMGB1-TLR4 signaling participates in renal ischemia reperfusion injury and could be attenuated by dexamethasone-mediated inhibition of the ERK/NF-κB pathway.

References for Histone H4 Rabbit pAb (A1131)

Product:

Histone H4 Rabbit pAb

Journal:

Human reproduction (Oxford, England)

Application:

IF:

6.35

Species:

Homo sapiens

PMID:

34647126

Title:

Decreased nitric oxide content mediated by asymmetrical dimethylarginine and protein l-arginine methyltransferase 3 in macrophages induces trophoblast apoptosis: a potential cause of recurrent miscarriage

References for Histone H4 Rabbit pAb (A1131)

Product:

Histone H4 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IHC

IF:

6.2

Species:

Rattus norvegicus

PMID:

36769358

Title:

Identification of Key Biomarkers Associated with Immunogenic Cell Death and Their Regulatory Mechanisms in Severe Acute Pancreatitis Based on WGCNA and Machine Learning - PMC

References for Histone H4 Rabbit pAb (A1131)

Product:

Histone H4 Rabbit pAb

Journal:

Molecular microbiology

Application:

IF:

3.97

Species:

Saccharomyces cerevisiae

PMID:

36760021

Title:

The interaction between the histone acetyltransferase complex Hat1‐Hat2 and transcription factor AmyR provides a molecular brake to regulate amylase gene …

References for Histone H4 Rabbit pAb (A1131)

Product:

Histone H4 Rabbit pAb

Journal:

Cell reports

Application:

IF:

Species:

Arabidopsis thaliana

PMID:

37393621

Title: