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Phospho-Histone H4-S1 Rabbit pAb (AP0901)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Phospho-Histone H4-S1 Rabbit pAb (AP0901)

Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0901) at 1:1000 dilution or Histone H4 Rabbit pAb (A1131). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or Nocodazole (50 ng/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-Histone H4-S1 Rabbit pAb
Catalog No.AP0901
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
ImmunogenA synthetic phosphorylated peptide around S1 of human Histone H4 (NP_003529.1).
SequenceMSGR
Gene ID
Swiss Prot
SynonymsH4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4A; Phospho-Histone H4-S1
Calculated MW11kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by Nocodazole
Cellular locationextracellular exosome, extracellular region, nucleoplasm, nucleus.

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ABclonal:Western blot - Phospho-Histone H4-S1 Rabbit pAb (AP0901)}

Western blot - Phospho-Histone H4-S1 Rabbit pAb (AP0901)

Western blot analysis of lysates from NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0901) at 1:1000 dilution or Histone H4 Rabbit pAb (A1131). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or Nocodazole (50 ng/mL) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (31)

Secondary Antibodies (26)