Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at 1:12000 dilution incubated overnight at 4℃. HeLa cells were treated by TSA (1 μM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of lysates from NIH/3T3 cells using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at 1:12000 dilution incubated overnight at 4℃. NIH/3T3 cells were treated by TSA (1 μM) at 37℃ for 18 hours.
Secondary antibody: () at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of lysates from C6 cells using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at 1:12000 dilution incubated overnight at 4℃. C6 cells were treated by TSA (1 μM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at 1:1000 dilution.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at a dilution of 1：6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at a dilution of 1：6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at a dilution of 1：6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at a dilution of 1：6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at a dilution of 1：6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunoprecipitation of Acetyl-Histone H4-K8 from 600 µg extracts of HeLa cells treated by TSA (1μM, 18h) was performed using 2 µg of Acetyl-Histone H4-K8 Rabbit mAb (A26921). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Acetyl-Histone H4-K8 Rabbit mAb (A26921) at a dilution of 1:5000.
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa, using 3 μg of Acetyl-Histone H4-K8 Rabbit mAb (A26921) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
| Product name | Acetyl-Histone H4-K8 Rabbit mAb |
|---|---|
| Catalog No. | A26921 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC70730 |
| Immunogen | A synthetic acetylated peptide around K8 of human Histone H4 (NP_003529.1). |
|---|---|
| Sequence | GGKGL |
| Gene ID | |
| Swiss Prot | |
| Synonyms | H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4A |
| Calculated MW | 11kDa |
| Observed MW | 11kDa |
| Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunoprecipitation |
| Positive samples | Acetyl K8, HeLa cells treated by TSA (1μM, 18h) |
| Cellular location | Chromosome, nucleus, Nucleus, Nucleus, extracellular exosome, extracellular region, nucleoplasm, nucleus. |
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* For research use only. Not for therapeutic or diagnostic purposes.
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