Review (1)Publications (7) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit pAb (A2370) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of various lysates using MonoMethyl-Histone H4-K20 Rabbit pAb (A2370) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H4-K20 antibody (A2370).
Immunohistochemistry analysis of paraffin-embedded Human colon using MonoMethyl-Histone H4-K20 Rabbit pAb (A2370) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis using MonoMethyl-Histone H4-K20 Rabbit pAb (A2370) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using MonoMethyl-Histone H4-K20 Rabbit pAb (A2370) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-K20 Rabbit pAb (A2370). Blue: DAPI for nuclear staining.
| Product name | MonoMethyl-Histone H4-K20 Rabbit pAb |
|---|---|
| Catalog No. | A2370 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic monomethylated peptide around K20 of human Histone H4 (NP_003529.1). |
|---|---|
| Sequence | HRKVL |
| Gene ID | |
| Swiss Prot | |
| Synonyms | H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; MonoMethyl-Histone H4-K20 |
| Calculated MW | 11kDa |
| Observed MW | 11kDa |
| Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | HeLa acid extract, NIH/3T3 acid extract, C6 acid extract, H4 protein |
| Cellular location | Chromosome, Nucleus. |
| Customer validation | (Mouse uterus) IF(Mus musculus) WB(Eurosta solidaginis, Homo sapiens, Mus musculus, Oryctolagus cuniculus) Co-IP(Mus musculus) |
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