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Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at 1:500 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K23 antibody (A18154) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameAcetyl-Histone H3-K23 Rabbit pAb
Catalog No.A18154
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic acetylated peptide around K23 of human Histone H3 (NP_003520.1).
SequenceATKAA
Gene ID
Swiss Prot
SynonymsH3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Acetyl-Histone H3-K23
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa treated by TSA, NIH/3T3 treated by TSA
Cellular locationChromosome, Nucleus.

Documents

Certificate of Compliance

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    ABclonal:Western blot - Acetyl-Histone H3-K23 Rabbit pAb (A18154)}

    Western blot - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

    Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at 1:500 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)}

    Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)}

    Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)}

    Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

    Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)}

    Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

    Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)}

    Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

    Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K23 Rabbit pAb (A18154)}

    Chromatin Immunoprecipitation - Acetyl-Histone H3-K23 Rabbit pAb (A18154)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K23 antibody (A18154) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

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