Western blot - Acetyl-Histone H3-K23 Rabbit pAb (A18154)
Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at 1:500 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry - Acetyl-Histone H3-K23 Rabbit pAb (A18154)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Acetyl-Histone H3-K23 Rabbit pAb (A18154) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - Acetyl-Histone H3-K23 Rabbit pAb (A18154)
Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K23 (A18154) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation - Acetyl-Histone H3-K23 Rabbit pAb (A18154)
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K23 antibody (A18154) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.