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MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of various lysates using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Dot Blot - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (A2358).

ABclonal:Immunohistochemistry - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Immunohistochemistry analysis of paraffin-embedded Human colon using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K9 antibody (A2358) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review

Overview

Product name	MonoMethyl-Histone H3-K9 Rabbit pAb
Catalog No.	A2358
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic monomethylated peptide around K9 of human histone H3 (NP_003520.1).
Sequence	ARKST
Gene ID
Swiss Prot
Synonyms	H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; MonoMethyl-Histone H3-K9
Calculated MW	16kDa
Observed MW	14kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 DB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 5μg-10μg of Chromatin ChIP-seq 1:20 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, NIH/3T3, C6
Cellular location	Chromosome, Nucleus.
Customer validation	(HeLa) WB(Mus musculus, Solanum lycopersicum, Musa nana Lour, Oryza sativa, Other) ChIP(Oryza sativa) IF(Mus musculus)

Documents

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Research Area

MonoMethyl-Histone H3-K9 Rabbit pAb images

Western blot - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Dot Blot - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K9 antibody (A2358).

Immunohistochemistry - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Immunofluorescence - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-K9 Rabbit pAb (A2358). Blue: DAPI for nuclear staining.

Immunofluorescence - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Chromatin Immunoprecipitation - MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A2358

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Reviews List

Product Protocols

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Cell Metabolism

Application:

IF:

27.287

Species:

Mus musculus

PMID:

30174306

Title:

Short-Term Mitochondrial Permeability Transition Pore Opening Modulates Histone Lysine Methylation at the Early Phase of Somatic Cell Reprogramming

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Cell reports

Application:

IF:

9.423

Species:

Mus musculus

PMID:

31291585

Title:

Branched-Chain Amino Acid Metabolic Reprogramming Orchestrates Drug Resistance to EGFR Tyrosine Kinase Inhibitors.

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Pharmacological Research

Application:

IF:

7.658

Species:

Mus musculus

PMID:

31830521

Title:

Lysine-specific demethylase-1 regulates fibroblast activation in pulmonary fibrosis via TGF-β1/Smad3 pathway

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

The New Phytologist

Application:

IF:

10.151

Species:

Solanum lycopersicum

PMID:

32255501

Title:

Histone demethylase SlJMJ6 promotes fruit ripening by removing H3K27 methylation of ripening-related genes in tomato

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Journal of microbiology (Seoul, Korea)

Application:

IF:

2.9

Species:

Mus musculus

PMID:

35437622

Title:

Gamma-glutamyltransferase of Helicobacter pylori alters the proliferation, migration, and pluripotency of mesenchymal stem cells by affecting metabolism and methylation status

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Food chemistry

Application:

IF:

9.23

Species:

Musa nana Lour

PMID:

36495744

Title:

Histone demethylase MaJMJ15 is involved in the regulation of postharvest banana fruit ripening

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

The Plant journal : for cell and molecular biology

Application:

IF:

7.09

Species:

Oryza sativa

PMID:

38116956

Title:

OsJMJ718, a histone demethylase gene, positively regulates seed germination in rice

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

38134595

Title:

Epigenetic modification of TWIST1 in macrophages promotes hypertension-induced atherosclerotic plaque instability

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Nature plants

Application:

ChIP

IF:

Species:

Oryza sativa

PMID:

35115727

Title:

The landscape of promoter-centred RNADNA interactions in rice

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Stem cell research & therapy

Application:

WB, IF

IF:

8.07

Species:

Mus musculus

PMID:

35715822

Title:

Glutamate regulates gliosis of BMSCs to promote ENS regeneration through -KG and H3K9/H3K27 demethylation - PMC

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Signal transduction and targeted therapy

Application:

IF:

38.1

Species:

Mus musculus

PMID:

37709738

Title:

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration - PMC

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Plant physiology

Application:

IF:

7.4

Species:

Other

PMID:

38669310

Title:

Histone H3K27 Demethylase SlJMJ3 Modulates Fruit Ripening in Tomato

References for MonoMethyl-Histone H3-K9 Rabbit pAb (A2358)

Product:

MonoMethyl-Histone H3-K9 Rabbit pAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

8.4

Species:

Mus musculus

PMID:

39148040

Title:

Gamma-glutamyl transferase secreted by Helicobacter pylori promotes the development of gastric cancer by affecting the energy metabolism and histone methylation status of gastric epithelial cells - PMC