Recombinant Human LEU3/CD4 Protein (RP00079)

Overview

Product name	Recombinant Human LEU3/CD4 Protein
Catalog No.	RP00079
Description	Recombinant Human LEU3/CD4 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys26-Trp390) of human sCD4 (Accession #NP_000607.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Human CD4 Protein at 1μg/mL (100 μL/well) can bind CD4 Rabbit mAb with a linear range of 0.006-0.18 ng/mL.
Purity	> 97% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	41.53 kDa

This protein is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system.

Product information

Synonym	CD4; CD4mut
Expression Host	HEK293 cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.