제품 > 항체 > 다중클론항체(pAb)

CD4 Rabbit pAb (A0363)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Rat lung, using CD4 Rabbit pAb (A0363) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from Mouse spleen, using CD4 Rabbit pAb (A0363) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from THP-1 cells, using CD4 Rabbit pAb (A0363) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - CD4 Rabbit pAb (A0363)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD4 Rabbit pAb (A0363) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded human spleen using CD4 Rabbit pAb (A0363) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of paraffin-embedded mouse spleen using CD4 Rabbit pAb (A0363) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	CD4 Rabbit pAb
Catalog No.	A0363
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 26-207 of CD4 (NP_000607.1).
Sequence	KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIV
Gene ID
Swiss Prot
Synonyms	T4; IMD79; Leu-3; OKT4D; CD4mut; CD4
Calculated MW	51kDa
Observed MW	51kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	THP-1, Mouse spleen, Rat lung
Cellular location	Cell membrane, Single-pass type I membrane protein.
Customer validation	IHC(Mus musculus) IF(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

CD4 Rabbit pAb images

Western blot - CD4 Rabbit pAb (A0363)

Immunofluorescence - CD4 Rabbit pAb (A0363)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for CD4 Rabbit pAb (A0363)

Product:

CD4 Rabbit pAb

Journal:

Frontiers in Cell and Developmental Biology

Application:

IHC

IF:

4.6

Species:

Mus musculus

PMID:

33330503

Title:

Human Umbilical Cord Blood Derived-Mesenchymal Stem Cells Alleviate Dextran Sulfate Sodium-Induced Colitis by Increasing Regulatory T Cells in Mice

References for CD4 Rabbit pAb (A0363)

Product:

CD4 Rabbit pAb

Journal:

Front Pharmacol

Application:

IHC

IF:

5.6

Species:

Mus musculus

PMID:

34483913

Title:

Aspirin Attenuates Cardiac Allograft Rejection by Inhibiting the Maturation of Dendritic Cells via the NF-κB Signaling Pathway

References for CD4 Rabbit pAb (A0363)

Product:

CD4 Rabbit pAb

Journal:

Acta biomaterialia

Application:

IF:

9.4

Species:

Mus musculus

PMID:

38185232

Title:

Biphasic calcium phosphate recruits Tregs to promote bone regeneration

References for CD4 Rabbit pAb (A0363)

Product:

CD4 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Homo sapiens,Mus musculus

PMID:

38778969

Title:

The therapeutic effect and targets of herba Sarcandrae on breast cancer and the construction of a prognostic signature consisting of inflammation-related genes - PMC

References for CD4 Rabbit pAb (A0363)

Product:

CD4 Rabbit pAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

Species:

Mus musculus

PMID:

38661278

Title:

Magnesium Ions Promote the Induction of Immunosuppressive Bone Microenvironment and Bone Repair through HIF1TGF Axis in Dendritic Cells

References for CD4 Rabbit pAb (A0363)

Product:

CD4 Rabbit pAb

Journal:

Cancer gene therapy

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38926596

Title:

Anti-angiogenesis and anti-immunosuppression gene therapy through targeting COUP-TFII in an in situ glioblastoma mouse model

References for CD4 Rabbit pAb (A0363)

Product:

CD4 Rabbit pAb

Journal:

Materials today. Bio

Application:

IF:

8.7

Species:

Homo sapiens

PMID:

39534677

Title:

Engineering exosomes from fibroblast growth factor 1 pre-conditioned adipose-derived stem cells promote ischemic skin flaps survival by activating autophagy - PMC