제품 > 항체 > 다중클론항체(pAb)

IκBα Rabbit pAb (A11397)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using IκBα Rabbit pAb (A11397) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Western blot analysis of lysates from Rat liver, using IκBα Rabbit pAb (A11397) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	IκBα Rabbit pAb
Catalog No.	A11397
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 201-300 of human IκBα (NP_065390.1).
Sequence	LLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTE
Gene ID
Swiss Prot
Synonyms	IKBA; MAD-3; NFKBI; EDAID2; IκBα
Calculated MW	36kDa
Observed MW	36kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IP 1:20 - 1:50
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	HeLa, NIH/3T3, Rat liver
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus, Mus musculus) IP(Rattus norvegicus) IHC(Rattus norvegicus)

Documents

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Research Area

IκBα Rabbit pAb images

Western blot - IκBα Rabbit pAb (A11397)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Inflammation

Application:

IF:

4.09

Species:

Mus musculus

PMID:

34341910

Title:

LncRNA H19 Mitigates Oxidized Low-Density Lipoprotein Induced Pyroptosis via Caspase-1 in Raw 264.7 Cells

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Biochem Pharmacol

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

33080187

Title:

Glutathione S-transferases P1-mediated interleukin-6 in tumor-associated macrophages augments drug-resistance in MCF-7 breast cancer

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Virus Res

Application:

IF:

3.303

Species:

Sus scrofa

PMID:

33713752

Title:

Porcine Circovirus Type 2 Infection Activates NF-κB pathway and Cellular Inflammatory Responses through circPDCD4/miR-21/PDCD4 Axis in Porcine Kidney 15 Cell

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Phytomedicine

Application:

IF:

5.34

Species:

Mus musculus

PMID:

34481339

Title:

Purslane (Portulacae oleracea L.) attenuates cadmium-induced hepatorenal and colonic damage in mice: Role of chelation, antioxidant and intestinal microecological regulation

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Exp Ther Med

Application:

IF:

2.447

Species:

Mus musculus

PMID:

34194560

Title:

Protective effects of miR?155?5p silencing on IFN?γ?induced apoptosis and inflammation in salivary gland epithelial cells

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Oxid Med Cell Longev

Application:

IF:

6.543

Species:

Homo sapiens

PMID:

34804360

Title:

Metformin Corrects Glucose Metabolism Reprogramming and NLRP3 Inflammasome-Induced Pyroptosis via Inhibiting the TLR4/NF-κB/PFKFB3 Signaling in Trophoblasts: Implication for a Potential Therapy of Preeclampsia

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Medicina (Kaunas, Lithuania)

Application:

IF:

2.94

Species:

Homo sapiens

PMID:

35744061

Title:

The Therapeutic Role of PNU-74654 in Hepatocellular Carcinoma May Involve Suppression of NF-κB Signaling - PMC

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

36577154

Title:

Nuciferine alleviates intestinal inflammation by inhibiting MAPK/NF-κB and NLRP3/Caspase 1 pathways in vivo and in vitro

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Pediatric research

Application:

IF:

3.95

Species:

Homo sapiens

PMID:

35523884

Title:

RRS1 knockdown inhibits the proliferation of neuroblastoma cell via PI3K/Akt/NF-κB pathway

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Journal of natural products

Application:

IF:

4.8

Species:

Mus musculus

PMID:

37002536

Title:

Synthesis of Lathyrol PROTACs and Evaluation of Their Anti-Inflammatory Activities

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Phytochemistry

Application:

IF:

Species:

Mus musculus

PMID:

36933878

Title:

Ent-kauranes and ent-atisanes from Euphorbia wallichii and their anti-inflammatory activity

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.67

Species:

Rattus norvegicus

PMID:

37237918

Title:

Biochanin A Ameliorates Nephropathy in High-Fat Diet/Streptozotocin-Induced Diabetic Rats: Effects on NF-kB/NLRP3 Axis, Pyroptosis, and Fibrosis - PMC

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

BMC complementary medicine and therapies

Application:

IF:

2.83

Species:

Homo sapiens

PMID:

37950195

Title:

Mechanism underlying the effect of Pulsatilla decoction in hepatocellular carcinoma treatment: a network pharmacology and in vitro analysis - PMC

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Aging

Application:

IF:

5.95

Species:

Homo sapiens

PMID:

37086261

Title:

Melatonin induces cell cycle arrest and suppresses tumor invasion in urinary bladder urothelial carcinoma - PMC

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Folia morphologica

Application:

WB, IP

IF:

1.19

Species:

Rattus norvegicus

PMID:

37957937

Title:

AEBP1 exacerbates myocardial ischemia-reperfusion injury via inhibition of IB

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.988

Species:

Homo sapiens, Mus musculus

PMID:

35359841

Title:

A Novel Modulator of the Renin-Angiotensin System, Benzoylaconitine, Attenuates Hypertension by Targeting ACE/ACE2 in Enhancing Vasodilation and Alleviating Vascular Inflammation

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Oncogene

Application:

IF:

8.75

Species:

Homo sapiens,Mus musculus

PMID:

38092960

Title:

RUNX1-IT1 acts as a scaffold of STAT1 and NuRD complex to promote ROS-mediated NF-B activation and ovarian cancer progression

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IHC

IF:

5.195

Species:

Rattus norvegicus

PMID:

28238827

Title:

Rhizoma Smilacis Glabrae inhibits pathogen-induced upper genital tract inflammation in rats through suppression of NF-B pathway

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

European journal of pharmacology

Application:

IF:

5.195

Species:

Mus musculus

PMID:

30716318

Title:

Harmine mitigates LPS-induced acute kidney injury through inhibition of the TLR4-NF-B/NLRP3 inflammasome signalling pathway in mice.

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Frontiers in oncology

Application:

IF:

5.73

Species:

Homo sapiens

PMID:

37361599

Title:

Determining the mechanism of pulsatilla decoction for treating gastric cancer: a network pharmacology-based study - PMC

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Rattus norvegicus

PMID:

35490493

Title:

Protective effects of E Se tea extracts against alcoholic fatty liver disease induced by high fat/alcohol diet: In vivo biological evaluation and molecular docking study

References for IκBα Rabbit pAb (A11397)

Product:

IκBα Rabbit pAb

Journal:

Military Medical Research

Application:

IF:

21.1

Species:

Mus musculus

PMID:

38937853