Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using Phospho-IκBα-S36 Rabbit mAb (AP0999) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight or treated by LPS (1 μg/mL) at 37℃ for 6 hours. Both NIH/3T3 cells and C6 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-IκBα-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
| Product name | Phospho-IκBα-S36 Rabbit mAb |
|---|---|
| Catalog No. | AP0999 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1543 |
| Immunogen | A synthetic phosphorylated peptide around S36 of human IκBα (P25963). |
|---|---|
| Sequence | LDSMK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | IKBA; MAD-3; NFKBI; EDAID2; Phospho-IκBα-S36 |
| Calculated MW | 36kDa |
| Observed MW | 39kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | THP-1 treated by LPS, THP-1 treated by PMA, NIH/3T3 treated by TNF-α, C6 treated by TNF-α |
| Cellular location | Cytoplasm, Nucleus. |
| Customer validation | WB(Homo sapiens, Mus musculus) IHC(Mus musculus) ELISA(Mus musculus) WB(Mus musculus) IHC(Mus musculus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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