Recombinant Human IkB-alpha/NFKBIA Protein (RP00032)

Overview

Product name	Recombinant Human IkB-alpha/NFKBIA Protein
Catalog No.	RP00032
Description	Recombinant Human IkB-alpha/NFKBIA Protein is produced by E. coli expression system. The target protein is expressed with sequence (Phe2-Leu317) of human IkB-alpha (Accession #NP_065390.1).
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Human IkB-alpha Protein at 2 μg/mL (100 μL/well) can bind IκBα Rabbit mAb with a linear range of 0.195-0.64 ng/mL.
Purity	> 90% by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	35.48 kDa

This protein is a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The protein is interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Product information

Synonym	IKBA; MAD-3; NFKBI; NFKBIA; MAD-3; NFKBI; IKB alpha; EDAID2; IKBA; NFKB inhibitor alpha
Expression Host	E. coli
Tag	No tag
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.