제품 > 항체 > 단일클론항체(mAb)

ABflo® 700 Rabbit anti-Human CD4 mAb (A26524)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 PC-3 cells (negative control, left) and THP-1 cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human CD4 mAb (A26524, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human CD4 mAb (A26524, 5 μl/Test, right).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 700 Rabbit anti-Human CD4 mAb (A26524, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human CD4 mAb (A26523, 5 μl/Test, right).Cells in the Monocyte gate were used for analysis.

Overview

Product name	ABflo® 700 Rabbit anti-Human CD4 mAb
Catalog No.	A26524
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC5142-01-ABf700

Background

This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Immunogen information

Immunogen	Recombinant protein of human CD4.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	T4; IMD79; Leu-3; OKT4D; CD4mut
Calculated MW	51kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein.

Research Area

ABflo® 700 Rabbit anti-Human CD4 mAb images

Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD4 mAb (A26524)

Flow cytometry: 1X10^6 THP-1 cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human CD4 mAb (A26524, 5 μl/Test, right).

Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD4 mAb (A26524)

* For research use only. Not for therapeutic or diagnostic purposes.