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DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

Western blot analysis of various lysates using DiMethyl-Histone H3-K14 Rabbit pAb (A5278) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Dot Blot - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (A5278) at 1:1000 dilution.

ABclonal:Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameDiMethyl-Histone H3-K14 Rabbit pAb
Catalog No.A5278
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic dimethylated peptide around K14 of human Histone H3 (NP_003520.1).
SequenceGGKAP
Gene ID
Swiss Prot
SynonymsH3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; DiMethyl-Histone H3-K14
Calculated MW16kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, Nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)}

    Western blot - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

    Western blot analysis of various lysates using DiMethyl-Histone H3-K14 Rabbit pAb (A5278) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Dot Blot - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)}

    Dot Blot - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

    Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K14 antibody (A5278) at 1:1000 dilution.
    ABclonal:Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)}

    Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

    Immunofluorescence analysis of C6 cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)}

    Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

    Immunofluorescence analysis of NIH-3T3 cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)}

    Immunofluorescence - DiMethyl-Histone H3-K14 Rabbit pAb (A5278)

    Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 pAb (A5278) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (105)

    Secondary Antibodies (26)