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Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from HeLa cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Dot Blot - Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421).

ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb
Catalog No.	A2421
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
Sequence	MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Gene ID
Swiss Prot
Synonyms	H3/j; H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FJ; H3C10; H3C11; HIST1H3J; Asymmetric DiMethyl-Histone H3-R17
Calculated MW	15kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa
Cellular location	Chromosome, Nucleus.
Customer validation	(Hippocampus) WB(Mus musculus, Eurosta solidaginis, Homo sapiens, Mus musculus) IF(Mus musculus)

Documents

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Research Area

Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb images

Western blot - Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Dot Blot - Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (A2421).

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421). Blue: DAPI for nuclear staining.

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Product:

Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb

Journal:

Journal of neuroscience

Application:

IF:

6.167

Species:

Mus musculus

PMID:

26377474

Title:

Arginine Methyltransferase 1 in the Nucleus Accumbens Regulates Behavioral Effects of Cocaine

References for Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Product:

Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb

Journal:

Journal of Surgical Research

Application:

IF:

2.192

Species:

Mus musculus

PMID:

29605032

Title:

GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice

References for Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Product:

Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Eurosta solidaginis

PMID:

37373302

Title:

Histone H3 and H4 Modifications Point to Transcriptional Suppression as a Component of Winter Freeze Tolerance in the Gall Fly Eurosta solidaginis - PMC

References for Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (A2421)

Product:

Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb

Journal:

Diabetes

Application:

IF:

9.33

Species:

Mus musculus

PMID:

37216643