제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Pig CD27 mAb (A23102)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Pig

Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F(Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Pig CD27 mAb(A23102, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).

Flow cytometry:1X10^6 293F cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Pig CD27 mAb(A23102, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Pig CD27 mAb
Catalog No.	A23102
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57399-ABf647

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Immunogen information

Immunogen	Recombinant protein of pig CD27.
Sequence	ATPAPQSCPEKHYWARGELCCQMCQPGTFLVKDCDQHGKAARCDPCKQGVSFSPDYHSRPHCESCRHCNSGLFVQNCTLTANAKCACPEGWQCRDKECTECDGPAQTPGPHPQPSHSPYAEAIPEARTSRYTQTLADVGQVPVPTISTYLSSQRCSSECIR
Gene ID
Swiss Prot
Synonyms
Calculated MW	27kDa
Observed MW

Applications

Reactivity	Pig
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	External side of plasma membrane.

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 647 Rabbit anti-Pig CD27 mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Pig CD27 mAb (A23102)

Flow cytometry:1X10^6 293F cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Pig CD27 mAb(A23102, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.