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ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)

Flow cytometry:1X10^6 293F cells (negative control, left) and 293F(Transfection, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)

Flow cytometry:1X10^6 293F(Transfection) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)

Flow cytometry:1X10^6 Human PBMC cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009, 5 μl/Test).

Overview

Product nameABflo® 488 Rabbit anti-Human IgG (Fc) mAb
Catalog No.A22504
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53383-ABf488
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to act upstream of or within several processes, including immunoglobulin mediated immune response; positive regulation of hypersensitivity; and positive regulation of phagocytosis. Located in extracellular space.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-330 of human IgG1 (Fc) (P01857).
SequencePKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Gene ID
Swiss Prot
Synonyms
Calculated MW36kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationCell membrane, Single-pass membrane protein, Secreted
Customer validation

IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)

    Flow cytometry:1X10^6 293F cells (negative control, left) and 293F(Transfection, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)

    Flow cytometry:1X10^6 293F(Transfection) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgG (Fc) mAb (A22504)

    Flow cytometry:1X10^6 Human PBMC cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test) or ABflo® 488 Rabbit anti-Human IgG (Fc) mAb(A22504, 5 μl/Test).The cells were simultaneously stained with ABflo® 647 Rabbit anti-Human/Monkey CD19 mAb(A23009, 5 μl/Test).

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A22504? Please let us know so that we can cite the reference in this datasheet.

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