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Human IgG Rabbit mAb (A19711)

Publications (17) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Human IgG Rabbit mAb (A19711)

Western blot analysis of lysates from Human plasma, using Human IgG Rabbit mAb (A19711) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Human IgG Rabbit mAb (A19711)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Human IgG Rabbit mAb (A19711) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameHuman IgG Rabbit mAb
Catalog No.A19711
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2241
Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to act upstream of or within several processes, including immunoglobulin mediated immune response; positive regulation of hypersensitivity; and positive regulation of phagocytosis. Located in extracellular space.
ImmunogenHuman IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW52kDa
Observed MW50kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:3000 - 1:18000
  • IHC-P 1:300 - 1:3000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive sampleshuman plasma
Cellular location
Customer validation

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Oryctolagus cuniculus, Arabidopsis thaliana)

ELISA(Homo sapiens)

ChIP(Homo sapiens, Other)

RIP(Drosophila melanogaster)

ChIP(Drosophila melanogaster)

Other(Mus musculus)

Co-IP(Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Human IgG Rabbit mAb (A19711)}

    Western blot - Human IgG Rabbit mAb (A19711)

    Western blot analysis of lysates from Human plasma, using Human IgG Rabbit mAb (A19711) at 1:3000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - Human IgG Rabbit mAb (A19711)}

    Immunohistochemistry - Human IgG Rabbit mAb (A19711)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil using Human IgG Rabbit mAb (A19711) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A19711? Please let us know so that we can cite the reference in this datasheet.

    항체 (9)

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    Secondary Antibodies (26)