제품 > 항체 > 2차 항체

HRP conjugated Rabbit-Mouse IgM (Fc) mAb (AS094)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP conjugated Rabbit-Mouse IgM (Fc) mAb (AS094)

Western blot analysis of lysates from Mouse IgM using HRP Rabbit anti-Mouse IgM mAb (AS094) at 1:50000 dilution.
Lysates/proteins: 50ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

Overview

Product nameHRP conjugated Rabbit-Mouse IgM (Fc) mAb
Catalog No.AS094
Host speciesRabbit
Purification methodAffinity purification
Isotype
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgM
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW55kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:50000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 50%glycerol,0.2%BSA,0.03%Proclin300,Negative IgG,pH7.3.
Key applicationWestern blotting    
Positive samplesMouse IgM
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - HRP conjugated Rabbit-Mouse IgM (Fc) mAb (AS094)}

    Western blot - HRP conjugated Rabbit-Mouse IgM (Fc) mAb (AS094)

    Western blot analysis of lysates from Mouse IgM using HRP Rabbit anti-Mouse IgM mAb (AS094) at 1:50000 dilution.
    Lysates/proteins: 50ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.

    * For research use only. Not for therapeutic or diagnostic purposes.