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[KD Validated] LAMP1/CD107a Rabbit mAb (A21194)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from U-251MG cells, using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells, using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - [KD Validated] LAMP1/CD107a Rabbit mAb (A21194)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] LAMP1/CD107a Rabbit mAb (A21194)

Confocal imaging of HeLa and HeLa-LAMP1-KD cells using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - [KD Validated] LAMP1/CD107a Rabbit mAb (A21194)

Flow cytometry: 1X10^6 knockdown (KD) HeLa cells (negative control, left) and HeLa cells (right) were intracellularly-stained with [KD Validated] LAMP1/CD107a Rabbit mAb (A21194, 2.5 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line), followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 HeLa cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or [KD Validated] LAMP1/CD107a Rabbit mAb (A21194, 2.5 μg/mL, right).

Overview

Product name	[KD Validated] LAMP1/CD107a Rabbit mAb
Catalog No.	A21194
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC52154

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 29-382 of human LAMP1/CD107a (NP_005552.3).
Sequence	AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM
Gene ID
Swiss Prot
Synonyms	LAMPA; CD107a; LGP120
Calculated MW	38kDa/45kDa
Observed MW	42kDa, 90-120kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:10000 - 1:40000 IHC-P 1:500 - 1:5000 IF/ICC 1:200 - 1:2000 FC (intra) 1:100 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	U-251 MG, HeLa
Cellular location	Cell membrane, Single-pass type I membrane protein, Endosome membrane, Lysosome membrane, Late endosome membrane, Cytolytic granule membrane.
Customer validation	WB(Homo sapiens) IF(Homo sapiens, Mus musculus)